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Twenty-nine thyroid tissue samples were collected from patients with thyroid nodules.The total RNA were extracted,the gene expressions of TFF3,HMGA2,C1orf24,and DDIT3 were detected by RT-PCR.In comparison with normal thyroid tissues,the expression of C1orf24 mRNA was decreased in the follicular thyroid adenoma (FTA) group,but increased in the follicular thyroid carcinomas (FTC) group.The expressions of DDIT3 and HMGA2 mRNA were increased in both FTA and FTC groups,and were even higher in the latter.The expressions of TFF3 mRNA level were decreased in FTA and FTC.The data suggested that molecular markers C1ort24,DDIT3,HMGA2,and TFF3 in thyroid tissue seem to be helpful in the differential diagnosis between follicular adenomas and carcinomas.
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Objective To investigate the clinical value of glypican-3 (GPC3) in the diaganosis of hepatocellular carcinoma (HCC),the contents of GPC3 in the serum and tissues of HCC patients were detected.Methods ELISA and immunohistochemical staining were applied to detect GPC3 expressing level in the serum and tissues in 79 cases with HCC,35 cases with post-hepatitis cirrhosis and 30 normal liver specimens and the resuits were compared.The influential factor of GPC3 content in the patients with HCC was analyzed by logistic regression model.Results The serum level of GPC3 in patients with HCC was (143.02±40.26) μg/L which was signifcantly higher than that in patients with post-hepatitis cirrhosis [(6.15±4.31) μg/L] and healthy controls [(4.47±3.22) μg/L] (all P ＜ 0.01).The expression levels of GPC3 was signifeantly higher in post-hepatitis cirrhosis tumor-adjacent tissue and tumor-distant tissue.The expression levels of GPC3 was significantly positively correlated with tumor presence of distant mestasis (x2 =13.182,P ＜ 0.0) and clinical stage (x2 =4.250,P ＜ 0.05),and not correlated with sex,age,tumor size and the level of AFP inserum (P ＜ 0.01).Conclusion GPC3 is specific the diagnosis of HCC.The joint diagnosis of GPC3 and AFP will improve the sensitivity of HCC.Therefore,GPC3 could as a biomarker for evaluating HCC condition and prognsis.
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Objective To discuss the efficiency and safety of transjugular intrahepatic portosystemic shunt(TIPSS) in treatment of cirrhotic patients with gastroesophageal variceal bleeding.Methods52 patients who had gastroesophageal variceal bleeding were treated with TIPSS,with 11 patients of them being active bleeding,23 patients being moderate to massive ascites.Results50 patients were treated successfully with the successful rate of 96.2%.The pressure of potal dropped from 3.87?0.50kPa to 2.45?0.40kPa(P
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patients with refractory ascites were treated 921 times using B type ascites concentrating device made in our institute.The mean time of reinfusion was(2 3?0 9)h and the average volume of ascites withdrawn from abdominal cacity was(6 820?2 315)ml.The weight was reduced(6 7?2 4)kg.The urine volume in 24 hours increased from (257 8?235 6)ml to (725 8?436 9)ml( P
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0.05). The median diameter of the nodules in survival group was smaller (4.3?2.2cm)than that in death group(7.3?2.7cm, P0.05). Ten of 14 survived patients remained clinical free of tumor. In conclusion, RFA could extend survival time for patients with unresectable PHC.